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. 2021 Feb 16;13(4):826. doi: 10.3390/cancers13040826

Figure 3.

Figure 3

EPI-X4 blocks the CXCR4 12G5 epitope on BCWM mutant cells and impairs migration. BCWM.1 cells were preincubated with 200 μM EPI-X4 or inactive control peptide and then stained with the anti-CXCR4 antibody clones 12G5 (A) or 1D9 (B). While EPI-X4 blocks binding of the anti-CXCR4 clone 12G5 to all CXCR4 variants tested, it did not affect binding of the 1D9 clone. Mean values (±SEM) of one exemplary experiment performed in triplicates are shown. (C) CXCL12-directed transwell migration of BCWM.1 cells expressing wildtype CXCR4 (WT non-transduced) or CXCR4 S338X was monitored in the presence or absence of EPI-X4 (200 µM). Mean values (± SEM) of three independent experiments performed in duplicates are shown (* p < 0.03 and ns, respectively).