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. 2021 Feb 16;10(4):793. doi: 10.3390/jcm10040793

Figure 1.

Figure 1

Identification of candidate antigens. (a) Western blot of pooled normal control (NC), lupus control (LC), proliferative lupus nephritis (PLN), and membranous lupus nephritis (MLN) sera (1:10,000 dilution) against human podocyte membrane proteins showing reactive band at approximately 50–60 kDa against 20 µg of podocyte membrane proteins in subjects with LN (previously reported in [13]). (b) Representative Western blots of NC, LC, PLN, and MLN sera (1:100) against 20 µg human glomerular extract (HGE) showing a region of interest between 40 and 55 kDa. (c) Identification of 102 overlapping glomerular (Glom) and podocyte (Pod) proteins using Scaffold software ver4.3.4 (Proteome Software, Portland, OR, USA).