Table 2.

The effect of size, poly dispersity index, encapsulation efficiency (%) and loading content (%), and zeta potential of the SF-CS nanoparticles after coating them with folic acid for the formation of SF-CS-FA nanoparticles. Optimal parameters are shown in bold.

Sample	CS: SF (mg/mL)	Folic Acid (g/L)	Size (nm)	PDI	EE%	LC%	Zeta Potentials (mV)
SF-CS-FA	5:3	0.1	45.8 ± 4.2	0.05	2.00 ± 0.0	0.0 ± 0.0	27.6 ± 1.6
		0.2	49.7 ± 7.2	0.09	7.9 ± 1.0	0.7 ± 0.5	21.1 ± 1.2
		0.3	54.5 ± 9.3	0.11	12.8 ± 1.3	9.2 ± 0.2	27.2 ± 2.1
		0.4	57.8 ± 10.2	0.16	13.7 ± 1.4	8.0 ±0.3	25.7 ± 1.3
		0.5	62.6 ± 12.9	0.13	15.8 ± 1.4	8.8 ± 1.7	37.4 ± 2.1
		0.6	61.5 ± 13.7	0.21	19.9 ± 1.9	7.56 ± 0.6	39.8 ± 0.7
		0.7	78.7 ± 11.1	0.20	49.9 ± 1.6	11.13 ± 1.1	38.5 ± 0.6
		0.8	81.7 ± 12.9	0.24	87.9 ± 1.1	19.9 ± 1.4	37.7 ± 1.4
		0.9	89.9 ± 10.6	0.26	88.9 ± 1.5	16.7 ± 1.6	36.4 ± 1.5
		1.0	91.5 ± 9.9	0.31	89.8 ± 1.6	17.9 ± 1.0	37.2 ± 0.3
		1.2	140.6 ± 9.7	0.50	76.2 ± 1.9	15.2 ± 1.5	27.4 ± 0.5
		1.4	165.8 ± 5.5	0.90	68.6 ± 1.1	11.8 ± 1.1	22.6 ± 1.7
		1.6	180.5 ± 10.1	1.89	55.9 ± 1.2	9.7 ± 0.7	23.8 ± 1.5
		1.8	289.9 ± 7.8	2.67	64.9 ± 0.3	7.7 ± 1.7	26.7 ± 0.5
		2.0	298.1 ± 5.9	3.83	75.6 ± 1.9	4.9 ± 1.2	29.7 ± 0.5