Fig 1. Gating of the monocyte subtypes.

Exclusion of air bubbles (plot 1), doublets (plot 2), CD45neg cells (plot 3), lymphocytes and granulocytes (plot 4), HLA-DR^neg cells (plot 5) and CD4^high cells (plot 6) in a first step with subsequent distinction of three monocyte subtypes based on the expression of CD14 and CD16 (plot 7). Abbreviations: SSC—sideward scatter, FSC—forward scatter, TOF—time of flight, PB—Pacific Blue, FITC—fluorescein isothiocyanate, APC–allophycocyanin, H7—Highlight 750, PE–phycoerythrin, Cy–cyanines, CD—cluster of differentiation protein, HLA-DR—human leucocyte antigen-antigen D related.