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. 2021 Feb 17;17(2):e1009307. doi: 10.1371/journal.ppat.1009307

Fig 4. Identification of the phosphorylation sites in chHDAC1 and 2.

Fig 4

(A) Amino acid sequence alignments of human HDAC1 and chicken HDAC1, and human HDAC2 and chicken HDAC2. Conserved serine (S) and threonine (T) sites are presented as bold underlined, and unique S and T sites are presented as bold italics (those marked with an * were mutated to alanine, A). The position of S and T in chicken HDAC1 and 2 are labeled below the amino acid sequences. pcDNA FLAG tagged MDV-1, MDV-2, or HVT US3, or pcDNA empty vector were co-transfected with pcDNA-FLAG-chHDAC1 (B) or pcDNA-FLAG-chHDAC2 mutants (C) into 293T cells. Forty-eight hours later, cells were lysed and subjected to Western blot with FLAG and HSP90 antibodies. Protein bands of expected phosphorylated chHDAC1 (p-chHDAC1) and p-chHDAC2 are marked by black arrow.