Fig 4. MTX treatment does not interfere with the mRNA expression of mPGES-1 enzyme in HSF infected with CHIKV or exposed to PIC or IFNβ.
A) mPGES-1 mRNA levels were assessed by qRT-PCR in HSF after CHIKV MOI 10−1 and 10−4 infection or PIC 100μg/mL and 10μg/mL stimulation in the presence and absence of MTX 1μM for 6h and 24h. B) mPGES-1 relative expression from HSF exposed to IFNβ 1000U/mL and 100U/mL +/- MTX 1μM for 6h and 24h as measured by qRT-PCR. mPGES-1 relative expression was also evaluated post-IL-1β 1ng/mL and TNFα 10ng/mL stimulation in the presence or not of DXM 1μM treatment for 6h and 24h. All experiments were done in triplicates. Results are expressed as mean ± standard error and presented as normalized fold increase vs control. *: p-values ≤ 0.05, **: p-values ≤ 0.01 and ***: p-values ≤ 0.001 represent significant difference from controls by one-way ANOVA followed by the Bonferroni’s test analysis. #: p-values ≤ 0.05, ##: p-values ≤ 0.01 signifies statistical difference of samples treated with the drug (MTX or DXM) compared to untreated samples under the different stimulatory conditions by Student’s t-test.