Figure 4.

Confocal images of a cross-section of top and bottom channels. The diagram (A) indicates the area of a cross-section where the image was taken. Z-projection of the membrane position with endothelial cells and pericytes on opposite sides of the membrane (B), and bottom position with endothelial cells (C). 3D view of the cross-section (D). ZO-1 tight junctions are stained in green. VE-cadherin adherens junctions are stained in red. Nuclei are stained in blue. Scale bars are 100 µm. The white dashed line in confocal images indicates the shadow coming from the wall of the channels.