Figure A3.

Schematic of cell-seeding areas in OoC device. (A) Top view of a μBBB device with an augmented image of the grid indicating an example of a top channel (vertical), bottom channel (horizontal), and a cross-section (indicated with a square in the centre). (B) Schematic of the transversal structure of each cross-section in the device. Pericytes are seeded in the top channel in contact with the polycarbonate membrane. Endothelial cells are seeded in the top channel, both in contact with the membrane and in contact with the bottom of the device. Each channel is supplied with its own cell-specific culture medium. The positions analysed for imaging purposes are indicated as bottom-position and membrane-position.