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. 2021 Feb 16;22(4):1959. doi: 10.3390/ijms22041959

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Histology of sperm and testes. (a). Adult testes were fixed in paraformaldehyde, sliced and colored with hematoxylin-eosin. (b). Papanicolaou staining of WT and KO sperm. (c). Sperm counts of WT and KO sperm. (d). Spontaneous acrosome reaction counting. There is no difference between WT and KO sperm in terms of acrosome reaction after capacitation. ns, not significant. (e). Analyses of sperm parameters by CASA. CASA analyzes of sperm movement parameters from Spata3 deleted mice showed no difference in comparison to those of WT sperm. As an example, here are presented: curvilinear velocity (VCL), beat/cross frequency (BCF) and amplitude of lateral head displacement (ALH). Data are represented as the mean ± SEM of at least three sperm samples from males of each group. (f). Schematic representation of CASA measurements.