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. 2021 Feb 16;22(4):1945. doi: 10.3390/ijms22041945

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Influence of Mycobacterium liposomes on HIV-1 cis-infection. Cells were infected with 8 ng CA-p24 of (A,B) pSG3-LAI (HIV-1 X4), (C,D) pSG3-BAL (HIV-1 R5) and pSG3Δenv (ΔpSG3) where (A,C) virus input with 100 ng of liposomes at the same time or (B,D) 100 ng of liposomes added to TZM-bl cells 30 min prior to adding virus. 48 h after infection cells were lysed to measure luciferase activity (relative light unit, RLU). The RLUs produced for each experiment were normalised to the average value of the negative control 0.8PC:0.2Ch liposomes. 0.8PC:0.2Ch is used here as a negative control and reference. For the data shown, n = 3. Mann–Whitney unpaired t-test was performed for (A–D).