Figure 6.
Role of H37Rv glycolipids in HIV-1 trans-infection via DC-SIGN. (A) H37Rv fractions 1 to 8 have been integrated into liposomes following the ratio 0.6PC:0.2Ch:0.2fraction and were tested on HIV-1 trans-infection via DC-SIGN in parallel of 0.8PC:0.2Ch and H37Rv liposomes. The data shown are from one experiment, n = 3. (B) H37Rv papA1Δ and H37Rv papA1Δ + SL1 were tested in parallel of 0.8PC:0.2Ch and H37Rv liposomes on HIV-1 trans-infection. The data shown are a pool of at least two independent experiments where n ≥ 6 in total. For (A,B) 0.5 × 106 Raji DC-SIGN were pre-incubated during 30 min with 100 μg liposomes or 50 µL of media. The cells were then incubated for 2 h with HIV-1 pseudo-typed virus (1) 12.5 ng CA-p24 pSG3-LAI (HIV-1 X4) or (2) 20 ng CA-p24 pSG3-BAL (HIV-1 R5). After capture the cells were washed and co-cultured with TZM-bl cells. The luciferase activity was read after 48 h. The RLUs produced for each experiment were normalised to the average value of the negative control 0.8PC:0.2Ch liposomes. Mann–Whitney unpaired t-test was performed for (A,B) and p-value represented with * for p-value < 0.05, ** for p-value < 0.01, *** for p-value < 0.001, **** for p-value < 0.0001.