Figure 9.

MRPL13 induces EMT process by activating the Akt/mTOR signaling pathway in MDA-MB-231 and T47D cell lines. MDA-MB-231 and T47D cell lines were transfected with siMRPL13-A (100 μM) for 72hr. (A) The protein level of EMT-related proteins (E-cad, Vimentin, Snail-1 and Snail-2) and AKT/mTOR pathway associated proteins (AKT, p-AKT, mTOR, p-mTOR) was detected by Western blot. For MRPL13 siRNA mediated knockdown rescue experiment, cells were transfected independently or jointly with the siMRPL13-A (100 μM) and PI3K/AKT/mTOR-specific agonist 740Y-P (20 μg/mL). The expression of mTOR signaling and EMT-related proteins was then assessed by Western blot in MDA-MB-231 (B) and T47D (C) cell lines. The β-actin served as a loading control for all above experiments. The above data are representative of three independent experiments.

Abbreviations: EMT, epithelial–mesenchymal transition; NC, negative control; E-cad, E-cadherin.