Table 1.
Gene | Primer Sequence |
---|---|
TNF-α | Forward: 5′-CGTCGTAGCAAACCACCAAG-3′ Reverse: 5′-GAGGCTGACTTTCTCCTGGT-3′ |
IL-1β | Forward: 5′-GGGATGATGACGACCTGCTA-3′ Reverse: 5′-TGTCGTTGCTTGTCTCTCCT-3′ |
Bcl-2 | Forward: 5′-CCTGGCATCTTCTCCTTCCA-3′ Reverse: 5′-GGACATCTCTGCAAAGTCGC-3′ |
Bax | Forward: 5′-AAGAAGCTGAGCGAGTGTCT-3′ Reverse: 5′-CCAGTTGAAGTTGCCGTCTG-3′ |
Caspase-3 | Forward: 5′-TGTCGATGCAGCTAACCTCA-3′ Reverse: 5′-GCAGTAGTCGCCTCTGAAGA-3′ |
GAPDH | Forward: 5′-TGTGAACGGATTTGGCCGTA-3′ Reverse: 5′-GATGGTGATGGGTTTCCCGT-3′ |
Abbreviations: TNF-α, tumor necrosis factor-a; IL-1β, interleukin-1β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.