Figure 2.

Activity-induced perinuclear PKA activity is mAKAPα dependent. Hippocampal neurons transfected with PN-AKAR4 and PN-RCaMP1h expression plasmids and infected with adenovirus for control or mAKAP shRNA were stimulated with 40 mm KCl; n = 15 for both shRNA and include data from three experiments using separate hippocampal neuron cultures. A, Averaged traces for PN-AKAR4. B, Amplitude of PN-AKAR4 traces. C, Averaged traces for PN-RCaMP1h. D, Amplitude of PN-RCaMP1h traces. Data in A, C are mean ± SEM; black bars in B, D indicate mean values. Datasets were normally distributed and compared by unpaired t tests; ***p ≤ 0.001.