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. 2021 Feb 23;8(1):ENEURO.0298-20.2021. doi: 10.1523/ENEURO.0298-20.2021

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Copyright © 2021 Boczek et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Perinuclear cAMP is regulated by L-type Ca²⁺ channels. Hippocampal neurons expressing PN-AKAR4 and PN-CaMP1h or AKAR4 and RCaMP1h were preincubated with 10 μm nifedipine (Nif; n = 19, 18 for parent and PN-sensors, respectively), 0.5 μm conotoxin GVIA (Con; n = 15, 36), or no inhibitor control (Ctrl; n = 14, 14) before stimulation with 40 mm KCl (bar). A, Averaged traces for PN-RCaMP1h. B, Amplitude of PN-RCaMP1h traces. C, Averaged traces for PN-AKAR4. D, Amplitude of PN-AKAR4 traces. E, Averaged traces for RCaMP1h. F, Amplitude of RCaMP1h traces. G, Averaged traces for AKAR4. H, Amplitude of AKAR4 traces. Traces show mean ± SEM and are normalized to initial baseline values (R₀ or I₀); black bars in B, D, F, H indicate mean values. Datasets were compared by Kruskal–Wallis and Dunn’s post hoc testing; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.