Figure 2.
Resting membrane properties remained unchanged after 6-OHDA injection. Electrophysiological passive properties for the data presented in Figure 1. A–C, Dopamine depleted hyperexcitable neurons did not shown significant changes in time constant τ (CON: 20.7 ± 2.97 ms, n = 12; 6-OHDA: 21.04 ± 3.66 ms, n = 13; t test, p > 0.94), membrane capacitance (CON: 112.6 ± 17.4 pF, n = 12; 6-OHDA: 117.0 ± 26.6 pF, n = 13; t test, p = 0.89), and Rin measured at VRest (Rin: CON: 166 MΩ [98.5,385.0], n = 17; 6-OHDA: 212.0 MΩ [125,476], n = 13; Mann–Whitney test, p = 0.19). Box and whisker plots represent medians (continous line), quartiles, and 5th–95th percentiles. The dotted protruding line indicates the mean of sample (for details, see Table 2). D, In the 6-OHDA mouse model, relationship between membrane capacitance and Rin is comparable to healthy cells in motor thalamus area. Box and whisker plots represent medians, quartiles, and 5th–95th percentiles. E–G, Histograms of data presented in panels A–C for controls (on top) and after treatment (bottom). Individual histograms represent distribution of τ, capacitance, and Rin data based on bin width 5 ms, 50 pF, and 20 MΩ, respectively.
Figure Contributions: Edyta K. Bichler conducted experiments, performed statistical analysis, and prepared figure. Dieter Jaeger reviewed data and analysis.