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. 2021 Feb 25;14:1261–1273. doi: 10.2147/OTT.S283548

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© 2021 Wang et al.

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The effects of ouabain on cell migration in A375 and SK-Mel-28 cells. (A) Melanoma cells were treated with indicated concentrations of ouabain for 12 hours, and the samples of A375 and SK-Mel-28 cells were then fixed and stained by crystal violet, the migration of melanoma cells were captured by inverted microscopy. The histograms of migration folds in A375 (B) and SK-Mel-28 (C) cells are shown. Wound closure analysis for A375 (D) and SK-Mel-28 (E) cells were explored at 12 hours and 24 hours, and the histograms of the percentage of wound closure for A375 (F) and SK-Mel-28 (G) cells at 24 hours are shown. The results are shown as mean±SD by Student’s t-test (two-tailed). *P<0.05, **P<0.01, and ***P<0.001.