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. 2021 Feb 22;2021:8869275. doi: 10.1155/2021/8869275

Table 1.

Temperature of the PCR condition.

Gene Predenaturation (°C/t) Denaturation (°C/t) Annealing (°C/t) Extension (°C/t) Final extension (°C/t) Cycle
PapC 95/3′ 95/30″ 62/30″ 72/40″ 72/5′ 35
FimA 95/5′ 95/30″ 62/30″ 72/30″ 72/5′ 35
SfaS 94/5′ 94/30″ 60/30″ 72/25″ 72/5′ 35