Table 3.
Effect of dietary supplementation with EtSF-S or FF on serum and hepatic glucose in ND- and HFFD-fed mice.
| Groups | Dose (%, w/w) | A: serum glucose (mmol/L) | B : hepatic glucose (µmol/g) | ||
|---|---|---|---|---|---|
| Fresh samples | Samples were stored for 24 h | ||||
| At −20°C | At 4°C | ||||
| ND-fed mice | – | ||||
| ND | 10 | 5.32 ± 0.59 | 147.27 ± 11.16 | 170.80 ± 26.23 | 341.64 ± 51.08## |
| ND/EtSF-S | 0.03 | 5.18 ± 0.65 | 123.12 ± 24.21 ∗ | 155.80 ± 17.43 | 357.66 ± 48.37## |
| ND/FF | 6.14 ± 0.50 ∗∗ | 82.22 ± 9.40 ∗∗ | 114.46 ± 7.60 ∗∗ | 215.15 ± 43.53∗∗## | |
|
| |||||
| HFFD-fed mice | – | ||||
| ND | 4.70 ± 0.80 | 160.42 ± 24.30 | 186.91 ± 21.70 | 260.22 ± 21.91## | |
| HFFD | 5.59 ± 1.07 ∗ | 142.28 ± 8.76 ∗ | 162.64 ± 13.66 ∗ | 251.56 ± 17.22## | |
| HFFD/EtSF-S | 1 | 5.33 ± 0.82 | 147.77 ± 17.6 | 170.71 ± 19.95 | 255.35 ± 28.42# |
| 5 | 6.21 ± 0.77 | 134.88 ± 6.85† | 150.64 ± 14.05 | 232.66 ± 11.78††## | |
| 10 | 6.58 ± 0.68† | 101.37 ± 20.02†† | 131.01 ± 8.18†† | 206.05 ± 39.86††## | |
| HFFD/FF | 0.03 | 6.03 ± 1.02 | 100.82 ± 14.41†† | 120.65 ± 13.45†† | 176.68 ± 33.35††## |
Mice were fed with normal diet (ND) or high fructose/fat diet (HFFD) for 3 months. Then, animals were fed with ND or HFFD without and with supplementation with the ethanol extract of Schisandrae Fructus (SF) seed (EtSF-S) or fenofibrate (FF). Thirty days later, serum and hepatic glucose was measured. In addition, the glucose levels of liver supernatants stored at 4°C or −20°C for 24 h were also determined for comparison. The concentrations of EtSF-S in chow diet were estimated on the basis of crude herbal material (SF seed). Values given are the mean ± SD, with N = 10. ∗p < 0.05, ∗∗p < 0.01 vs. ND alone; †p < 0.05, ††p < 0.01 vs. HFFD alone, ##p < 0.01 vs. the sample stared at −20°C.