Figure 2.

Baseline differential levels of histone demethylase JMJD2B and downstream targets in healthy and asthmatic fibroblasts. (a) qRT-PCR analysis of JMJD2B expression levels. (b) Western bolt analysis showing JMJD2B and trimethylated histones H3 lysine residues at K4, K9, K27, K36, and K79 protein levels in the fibroblasts. (b) Calculated mean ± SD fold change in protein-expression levels in normal and asthma fibroblasts based on two separate experiments. ^∗∗∗p < 0.01, determined using unpaired two-tailed Student t-test. Representative immunoblots depicting protein levels normal and asthma fibroblasts where β-actin was used as loading control.