TABLE 1.
Illumina adapters and primers (sequence 5′‐3′) from the literature used in HTS for COI, 16S, and ITS
| Adapter overhang sequences | ||
| Forward overhang | 5′ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG‐[locus‐specific sequence] | “16S Metagenomic Sequencing Library Preparation” (2013) |
| Reverse overhang | 5′ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG‐[locus‐specific sequence] | |
| COI Primers | ||
| MLepF1 | GCTTTCCCACGAATAAATAATA | Hajibabaei et al. (2006) |
| RonMWASPdeg | GGWTCWCCWGATATAKCWTTTCC | M. A. Smith (unpublished); Hernández‐Triana et al. (2014) |
| LepR1 | TAAACTTCTGGATGTCCAAAAAATCA | Hebert et al. (2004); Hernández‐Triana et al. (2014) |
| HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | Folmer et al. (1994) |
| 16S Primers | ||
| 16S Forward | CCTACGGGNGGCWGCAG | “16S Metagenomic Sequencing Library Preparation” (2013) |
| 16S Reverse | GACTACHVGGGTATCTAATCC | |
| ITS Primers | ||
| ITS86_F | GTGAATCATCGAATCTTTGAA | Beeck et al. (2014) |
| ITS4_R | TCCTCCGCTTATTGATATGC | |
COI primers have been used as cocktails, combining 1 to 1 volume of the two forwards (forward cocktail) and the two reverse (reverse cocktail) primers. References are also shown.