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. 2021 Mar 2;34:102372. doi: 10.1016/j.nano.2021.102372

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Analysis of the anti-S_461-493 IgG subclass abundance in test mice. Mice groups were s.c.-immunized on days 0, 14, and 21 with: 5 μg of the S_461-493 peptide diluted in PBS, the S_461-493 peptide plus complete Freund's adjuvant (+FA; for priming, while for boosting the incomplete version was used), and a low (L) or a high (H) dose of AuNP-S_461-493, corresponding to 0.5 and 5 μg of S_461-493, respectively. Anti-S_461-493 IgG1 or IgG2 seric levels were determined on by ELISA at a 1:200 dilution. The asterisk indicates statistically significant differences versus the IgG2a levels.