Figure 3.

miRNA-92a-3p promotes osteoblastogenesis and matrix mineralization

Transfection of agomiRNA-92a-3p, antagomiRNA-92a-3p, agomiRNA-NC, antagomiRNA-NC, and Lipofectamine 3000 control (200 μm) in MC3T3-E1 cells for 48 h. (A) RT-PCR showed that mmu-miRNA-92a-3p expression was upregulated in agomiRNA-92a-3p. (B) RT-PCR showed that OCN expression was upregulated in agomiRNA-92a-3p. (C) RT-PCR showed that Runx2 expression was upregulated in agomiRNA-92a-3p. (D) RT-PCR showed that Alkaline phosphatase staining (ALP) expression was upregulated in agomiRNA-92a-3p. (E) RT-PCR showed that mRNA expression was upregulated in agomiRNA-92a-3p. (F) Western blotting to evaluate the expression of OCN, Runx2, ALP, and Col1a1 protein after 48 h. (G) ALP after 14 days (scale bar, 10 mm). (H) Alizarin red-mediated calcium staining after 21 days. n = 3; data are presented as mean ± SD (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; # no significance).