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. 2021 Feb 15;23:1345–1359. doi: 10.1016/j.omtn.2021.02.008

Table 2.

mRNA and miRNAs primer sequences

miRNA or gene name Primer sequence (5′ to 3′)
mmu-miRNA-92a-3p-forward 5′-TTCACTTGTCCCGGCCTGT-3′
mmu-miRNA-92a-3p- reverse 5′-CTCAACTGGTGTCGTGGAGTC-3′
hsa-miRNA-92a-3p-forward 5′-TTCACTTGTCCCGGCCTGT-3′
hsa-miRNA-92a-3p- reverse 5′-CTCAACTGGTGTCGTGGAGTC-3′
mmu-miR-U6-forward 5′-CTCGCTTCGGCAGCACAT-3′
mmu-miR-U6-reverse 5′-AACGCTTCACGAATTTGCGT-3′
hsa-IBSP-forward 5′-GAACCTCGTGGGGACAATTAC-3′
hsa-IBSP-reverse 5′-CATCATAGCCATCGTAGCCTTG-3′
mmu-IBSP-forward 5′-ACCTCGCCACCGATCAGAA-3′
mmu-IBSP-reverse 5′-TGTTGTATTCGTTGCCTGTTTGT-3′
hsa-ALP-forward 5′-ACTGGGGCCTGAGATACCC-3′
hsa-ALP-reverse 5′-TCGTGTTGCACTGGTTAAAGC-3′
mmu-ALP -forward 5′-GGCAAAGAGGGAGCTAGAA-3′
mmu-ALP-reverse 5′-ATGGCCGTGCAGATGTA-3′
hsa-COL1A1-forward 5′-GAGGGCCAAGACGAAGACATC-3′
hsa-COL1A1-reverse 5′-CAGATCACGTCATCGCACAAC-3′
mmu-COL1A1 -forward 5′-CAGAGGCGAAGGCAACA-3′
mmu-COL1A1-reverse 5′-GTCCAAGGGAGCCACATC-3′
hsa-OCN-forward 5′-GGCGCTACCTGTATCAATGG-3′
hsa-OCN-reverse 5′-GTGGTCAGCCAACTCGTCA-3′
mmu-OCN -forward 5′-GCTGTTTGTTCGGGTCTC-3′
mmu-OCN-reverse 5′-GGGCCAAAGTCAGCATC-3′
hsa-RUNX2-forward 5′-TGGTTACTGTCATGGCGGGTA-3′
hsa-RUNX2-reverse 5′-TCTCAGATCGTTGAACCTTGCTA-3′
mmu-RUNX2 -forward 5′-GCCGGGAATGATGAGAAC-3′
mmu-RUNX2-reverse 5′-TGGGGAGGATTTGTGAAGA-3′
mmu-GAPDH-forward 5′-AGGTCGGTGTGAACGGATTTG-3′
mmu-GAPDH-reverse 5′-GGGGTCGTTGATGGCAACA-3′
hsa-GAPDH-forward 5′-GGAGCGAGATCCCTCCAAAAT-3′
hsa-GAPDH-reverse 5′-GGCTGTTGTCATACTTCTCATGG-3′