Figure 7.

CDK5 overexpression inhibited the therapeutic effects of miR-188-3p-enriched exosomes

(A) RT-PCR results of CDK5 in control, vector, and CDK5-overexpression groups. (B) Western blot results of CDK5 in each group. (C) EdU assay results in control, MPP+, MPP+ with miR-188-3p-enriched exosomes, and MPP+ with miR-188-3p exosomes + CDK5-overexpressed vectors groups. (D) Quantitative analyses of an EdU assay. (E and F) Flow cytometry results of the apoptosis rate in MN9D cells. (G) Western blot results of CDK5, LC3-I, LC3-II, and p62 in each group. (H) Quantitative analyses of western blot results. (I) Immunofluorescence analysis for LC3B in each group. (J) Quantitative analyses for immunofluorescence results. (K) TEM photographs verified the level of autophagy. (L) Quantitative analyses of TEM photos. (M) Western blot results of NLRP3, ASC, Casp1 p20, and Procasp1 in each group. (N) Quantitative analyses of western blot results. Data are reported as mean ± SD, n = 3. ∗∗∗p < 0.001.