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. 2021 Feb 10;23:1304–1322. doi: 10.1016/j.omtn.2021.02.005

Figure 4.

Figure 4

LINC00174 interacts with SRSF1

(A) Schematic illustration of RNA pulldown assay.

(B) Mouse primary myocardial cells were lysed and incubated with biotinylated LINC00174 sense or antisense strand; pulldown samples were then analyzed by western blotting.

(C) Mouse primary myocardial cells were lysed and incubated with indicated antibodies in RIP assay. Immunoprecipitated (IP)-enriched RNA was then analyzed by qRT-PCR; n = 5.

(D) Schematic illustration of SRSF1 full-length protein and truncations; their expression in E. coli was shown in the SDS-PAGE gel below.

(E) The binding of LINC00174 to SRSF1 full-length protein and truncated proteins was assessed by RIP.

(B–E) The data represented 1 of 3 independent experiments. (C and E) Data were represented as means ± SDs. p values were determined by 1-way ANOVA), followed by Tukey post hoc test. ∗p < 0.05 and ∗∗p < 0.01.