Figure 5.

LINC00174 regulates p53 signaling through SRSF1

(A and B) Mouse primary myocardial cells were pretreated as indicated before H/R treatment. The relative expression of SRSF1 after H/R treatment for 4 h was measured by qRT-PCR; n = 3.

(B) The expression of SRSF1 after H/R treatment was measured by western blotting. β-Actin was used as the loading control.

(C) Mouse primary myocardial cells were transfected with indicated plasmids or siRNAs; 48 h later, the expression of p53 was measured by western blotting. β-Actin was used as the loading control.

(D–F) Mouse primary myocardial cells were pretreated as indicated. Then the cells were subjected to H/R treatment for 4 h.

(D) The apoptosis of myocardial cells was determined by TUNEL assay; cell nuclei were stained by DAPI; merged images are shown at the bottom. Scale bar, 200 μm.

(E) The level of LC3 in myocardial cells was examined by immunohistochemical staining. Scale bar, 10 μm.

(F) The expression of autophagy-related proteins, SRSF1, and p53 in myocardial cells was measured by western blotting; β-actin was used as the loading control.

(A–F) The data represented 1 of 3 independent experiments. (A) Data were represented as means ± SDs. p values were determined by 1-way ANOVA, followed by Tukey post hoc test; ∗∗p < 0.01.