Table 1.
Common RI matching solutions
| Type | Chemicals | RI | Clearing methods or cocktails |
|---|---|---|---|
| Hydrophilic reagents | Glycerol | 1.48 | Scale, CLARITY, ScaleS, SOCW, FOCM |
| Fructose | 1.49 | SeeDB, FRUIT | |
| Sucrose | 1.44 | CUBIC, UbasM | |
| Sorbitol | 1.47 | SOCS, sRIMS, DMSO/Sorbitol, ScaleS, MACS, FOCM | |
| Xylitol | 1.39 | ClearSee, ePro-ClearSee | |
| Polyethylene glycol | 1.46–1.47 | ClearT2, PEGASOS | |
| Iohexol | – | RIMS, Ce3D, SeeDB2 | |
| Iodixanol | 1.41 | SWITCH | |
| Diatrizoic acid | – | FocusClear, Stochastic electrotransport | |
| Antipyrine | 1.59 | CUBIC-R, CUBIC-RA | |
| N-methylnicotinamide | 1.58 | CUBIC-R, CUBIC-RA | |
| Nicotinamide | 1.47 | CUBIC-R, CUBIC-R+ | |
| N-methyl-d-glucamine | – | Stochastic electrotransport, SWITCH | |
| DMSO | 1.48 | DMSO/Sorbitol, ScaleS, FOCM | |
| TDE | 1.52 | TDE/PBS, TOMEI, LUCID, CLARITY | |
| Phosphoric acid | 1.43 | Phosphoric acid (8.5–14.2 M) | |
| Hydrophobic reagents | Methyl salicylate | 1.54 | Spalteholz's technique |
| Benzyl alcohol | 1.54 | BABB, uDISCO, vDISCO | |
| Benzyl benzoate | 1.57 | BABB, uDISCO, vDISCO, PEGASOS | |
| DBE | 1.56 | 3DISCO, iDISCO, iDISCO+, FDISCO, Adipo-Clear, CalAdipoClear | |
| DPE | 1.58 | uDISCO | |
| ECi | 1.56 | Ethanol-ECi, 2ECi | |
| Resin | 1.56 | CRISTAL |