Fig. 3.

Ag85B detection in transgenic alfalfa lines by ELISA analysis using serum from a naturally infected animal with MAP for labeling. The absorbance from the near isogenic control alfalfa (WT) was subtracted (withdraw) from the values of the transgenic alfalfa lines. Statistical differences between transgenic alfalfa lines and WT are denoted by an asterisk (*). Alphanumeric keys were assigned to each transgenic plant by adding 85L and the progressive number of obtained lines from 1 to 20