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Antigenicity of the alfalfa-made Ag85B. ELISA was applied to transgenic alfalfa line (#20) expressing the highest amount of recombinant Ag85B. Primary labeling was performed with sera from animals naturally infected with MAP (1:25 dilution; provided by the Autonomous University of Baja California, Mexicali, Baja California, México). Protein samples from near isogenic control alfalfa plants (WT) were labeled with serum from the MAP-infected animal #8
