Fig. 6.

Anti-85B antibody levels in sera (a, IgG) and feces (b, IgA) from orally treated mice with wild type (WT) or transgenic alfalfa expressing Ag85B plus CT adjuvant (85B + CT). BALB/c mice were immunized on days 0, 7, 15, and 24 with 100 mg wild type alfalfa in PBS by the oral route (i), or 100 mg transgenic alfalfa expressing Ag85B in PBS plus cholera toxin (10 μg CT, Sigma) by the oral route (ii). Antigen-specific total immunoglobulin G a and A b levels were measured in serum and feces samples by ELISA. Purified Ag85B antigen was used to coat ELISA plates (1 μg per well)