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. 2020 Oct 10;24(1):57–65. doi: 10.1007/s10456-020-09751-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–d 2D slices of 3D raw image and binarization of two scans. 1810 × 1810 voxels, voxel size 325 nm, physical size about 590 μm × 590 μm. e Workflow for the extraction of vessels with diameter larger than 30 μm. f Visualization of observation window W