Table 5.
The mRNA expression levels of tight junctions and inflammatory cytokines in jejunum of Beijing You Chickens.
| Items | Group |
SEM | Main effect |
P-value |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FB | FG | CB | CG | Basal | GOD | Litter floor | Cage | GOD | Housing systems | Housing systems × GOD | ||
| 42d | ||||||||||||
| Occludin | 1.00 | 1.18 | 0.89 | 0.81 | 0.050 | 0.95 | 1.00 | 1.09a | 0.85b | 0.598 | 0.016 | 0.169 |
| Claudin-1 | 1.00 | 0.99 | 1.13 | 0.98 | 0.092 | 1.06 | 0.99 | 0.99 | 1.06 | 0.691 | 0.753 | 0.729 |
| ZO-1 | 1.00 | 0.99 | 1.12 | 1.03 | 0.067 | 1.06 | 1.01 | 1.00 | 1.07 | 0.716 | 0.588 | 0.749 |
| Mucin-2 | 1.00 | 1.02 | 0.66 | 0.58 | 0.056 | 0.83 | 0.80 | 1.01a | 0.62b | 0.692 | <0.001 | 0.587 |
| IL-1β | 1.00 | 1.27 | 1.00 | 1.32 | 0.105 | 1.00 | 1.30 | 1.13 | 1.16 | 0.173 | 0.903 | 0.884 |
| IL-4 | 1.00 | 0.88 | 0.98 | 1.29 | 0.065 | 0.99 | 1.09 | 0.94 | 1.13 | 0.438 | 0.142 | 0.101 |
| TLR-4 | 1.00 | 0.94 | 1.13 | 1.06 | 0.075 | 1.07 | 1.00 | 0.97 | 1.09 | 0.677 | 0.423 | 0.984 |
| IFN-γ | 1.00 | 1.14 | 1.21 | 0.97 | 0.132 | 1.10 | 1.05 | 1.07 | 1.09 | 0.851 | 0.954 | 0.498 |
| TNF-α | 1.00 | 1.00 | 0.93 | 0.88 | 0.037 | 0.96 | 0.94 | 1.00 | 0.90 | 0.790 | 0.195 | 0.752 |
| 77d | ||||||||||||
| Occludin | 1.00b | 1.68a | 1.65a | 1.43a | 0.073 | 1.32 | 1.55 | 1.34 | 1.54 | 0.050 | 0.095 | 0.001 |
| ZO-1 | 1.00b | 1.68a | 1.89a | 1.42a,b | 0.123 | 1.45 | 1.55 | 1.34 | 1.66 | 0.642 | 0.173 | 0.017 |
| Mucin-2 | 1.00 | 0.91 | 1.61 | 1.30 | 0.077 | 1.31 | 1.10 | 0.96b | 1.45a | 0.115 | <0.001 | 0.373 |
| IL-1β | 1.00 | 1.47 | 0.91 | 1.04 | 0.113 | 0.96 | 1.25 | 1.23 | 0.97 | 0.195 | 0.251 | 0.447 |
| IL-4 | 1.00 | 1.20 | 1.39 | 0.95 | 0.089 | 1.20 | 1.07 | 1.10 | 1.17 | 0.479 | 0.686 | 0.073 |
| TLR-4 | 1.00 | 1.49 | 1.09 | 1.43 | 0.065 | 1.05b | 1.46a | 1.25 | 1.26 | 0.001 | 0.892 | 0.505 |
| IFN-γ | 1.00 | 1.75 | 1.21 | 1.15 | 0.134 | 1.10 | 1.45 | 1.38 | 1.18 | 0.199 | 0.454 | 0.132 |
| TNF-α | 1.00 | 0.95 | 1.64 | 1.55 | 0.074 | 1.32 | 1.25 | 0.97b | 1.60a | 0.490 | <0.001 | 0.852 |
a–bMeans in the same row without the same superscript differ significantly (P < 0.05). “+” means add. SEM means standard error of the mean. (n = 8).
Abbreviations: CB, rear in the cage fed with basal dietary; CG, rear in the cage fed with GOD; FB, rear on the litter floor fed with basal dietary; FG, rear on the litter floor fed with GOD dietary; GOD, glucose oxidase.