Supplementary Figure 4.

CAMYEL cAMP sensor evidenced lack of saturation at 10 μM of GLP-1R agonist. Time course (A) and fitted dose-response curve (B) of cAMP-positive control forskolin as measured by the intracellular sensor CAMYEL in HEK293T cells. Stimulation with 10 μM of agonist or forskolin in GLP-1R+ HEK293T cells (C). The +iAUC representation of vehicle and baseline-corrected temporal response to each agonist is expressed as mean ± SE. Three independent experiments were performed with at least two technical replicates per group.