Figure 3.

Dose-dependent effects of ligands on Gα_srecruitment and cAMP production. Dose–response curves (A) and temporal resolution (1 μM stimulation) (B) of ligand-induced BRET changes between Nluc-tagged Gα_s recruitment to GFP-tagged GLP-1R. Dose–response curves of ligand-induced cAMP production GLP-1R⁺ HEK293T cells (C). Dose–response curves (D) and temporal resolution (1 μM stimulation) (E) of ligand-induced Gα_s recruitment to the GIPR. Dose–response curves of ligand-induced cAMP production GIPR⁺ HEK293T cells (F). +iAUC representation of vehicle and baseline-corrected 60 min (Gα_s recruitment) or 25 min (cAMP generation) temporal responses to each agonist is expressed as mean ± SEM. Three independent experiments were performed with at least two technical replicates per group.