Figure 5.

Ligand-induced GIPR internalization. Dose–response curves (A) and temporal resolution (1 μM stimulation) (B) of ligand-induced hGIPR-Rluc8 internalization. Live HILO imaging of GIPR-GFP internalization in HEK293T cells at baseline and approximately 15 min after drug (1 μM) treatment (C). Dose–response curves for β-arrestin 2-Rluc8 (D) and Gα_q-Nluc recruitment (E). The + iAUC representation of vehicle and baseline-corrected 20 min (GIPR internalization), 30 min (β-arrestin1/2 recruitment), or 60 min (Gα_q recruitment) temporal response to each agonist is expressed as mean ± SEM. Three independent experiments were performed with at least two technical replicates per group.