Fig. 2. Piezo1 expression and Piezo1-dependent calcium influx in HDFs subjected to CMS.

A Expression of Piezo1 in HDFs was determined by western blotting in a strength dependent manner. B Quantitative analysis of the expression level of Piezo1. C Expression of Piezo1 in HDFs was determined by western blotting in a time-dependent manner. D Quantitative analysis of the expression level of Piezo1. E Representative fluorescence images of intracellular Ca²⁺ in HDFs. (Scale bar: 100 μm). F Mean fluorescence intensity quantification of Ca²⁺ levels in HDFs. G Representative fluorescence images of intracellular Ca²⁺ in HDFs in the context of Piezo1 knockdown. (Scale bar: 100 μm). H Mean fluorescence intensity quantification of Ca²⁺ levels in HDFs in the context of Piezo1 knockdown. Ca²⁺ concentration assay in HDFs was performed in the condition of mechanical stretch (10%, 1 h) and GsMTx4 exposure (1 h). The results are expressed as the means with SD (n = 3). One-way ANOVA is used for all analysis. *P < 0.05, **P < 0.01, ***P < 0.005.