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. 2021 Mar 1;7:41. doi: 10.1038/s41420-021-00419-x

Fig. 2. TINCR promotes breast cancer cell proliferation and metastasis in vitro.

Fig. 2

A TINCR expression was examined in normal mammary epithelial cell line (MCF10A) and breast cancer cell lines by qRT-PCR assay. B Left: The relative expression of TINCR in T47D and MCF-7 cells transfected with scrambled, si-TINCR 1# and si-TINCR 2#; right: the relative expression of TINCR in T47D and MCF-7 cells transfected with empty vector and pcDNA-TINCR. C, D CCK-8 assays were performed to determine the viability of T47D and MCF-7 cells treated with si-TINCR or pcDNA-TINCR. E, F Colony formation assays were used to detect the proliferation of si-TINCR-transfected or pcDNA-TINCR-transfected T47D and MCF-7 cells. Colonies were counted and captured. G, H Proliferous breast cancer cells were displayed by EdU immunostaining assays. EdU positive cells were counted and captured (400×). I, J Transwell assays were performed to determine migration in breast cancer cells treated with si-TINCR or pcDNA-TINCR. Values are shown as the mean±s.d in three independent experiments. The mean values of two groups were compared via paired, two-tailed Student t tests. *P<0.05, **P<0.01.