Fig. 4. Effects of TINCR on breast cancer tumorigenesis in vivo.

A Empty vector or sh-TINCR was transfected into MCF-7 cells, which were injected in the BALB/c-nude mice (n=7), respectively. Tumors before and after carrying from the nude mice. B Tumor volumes were calculated after injection every five days. Points, mean (n=7); bars indicate SD. C Tumor weights were represented as means of tumor weights±SD. D qRT-PCR was used to detect the average expression of TINCR in xenograft tumors (n=7). E The xenograft tumor sections were under H&E staining and immunohistochemistry staining using antibodies against ki-67 (200×). Values are shown as the mean±s.d in three independent experiments. The mean values of two groups were compared via paired, two-tailed Student t tests.*P<0.05, **P<0.01.