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. 2021 Mar 1;7:41. doi: 10.1038/s41420-021-00419-x

Fig. 5. TINCR can bind to cytoplasmic protein STAU1.

Fig. 5

A, B qRT-PCR assays were performed to reveal the expression levels of TINCR in the cytoplasm or nucleus of T47D and MCF-7 cells. GAPDH was used as a cytosol marker and U6 was used as a nuclear marker. C RIP assay was performed with IgG or STAU1 antibodies in T47D cells, and the coprecipitated RNA was subjected to qRT-PCR for TINCR. D qRT-PCR assay examined TINCR expression in breast cancer cells transfected with control (scrambled), si-STAU1 1# and si-STAU1 2#. E qRT-PCR assay examined STAU1 expression in breast cancer cells treated with si-TINCR or pcDNA-TINCR. F Western blot assay examined STAU1 expression in breast cancer cells treated with si-TINCR or pcDNA-TINCR. Values are shown as the mean ± s.d in three independent experiments. The mean values of two groups were compared via paired, two-tailed Student t tests.*P < 0.05, **P < 0.01.