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Enzyme kinetic parameters.
| Enzyme | Substrate | Km (mM) | Vmax (µmol min−1 mg−1) | Kcat (s−1) |
|---|---|---|---|---|
| Two-domain WT | Urocanate | 0.22 ± 0.03 | 1.71 ± 0.12 | 1.43 |
| Fumarate | n.d. | n.d. | n.d. | |
| Full-length WT | Urocanate | 0.43 ± 0.03 | 5.57 ± 0.18 | 5.62 |
| Fumarate | n.d. | n.d. | n.d. |
Data are presented as mean +/− SEM.
