Fig. 3.

Puerarin protects against OVX plus AAC-induced cardiac hypertrophy through upregulation of the PPARα/PGC-1 pathway. a Representative Western blots of whole lysates and fold changes in the relative densitometric values of PPARα and PPARγ in hearts obtained from the indicated groups. b Representative Western blots of whole lysates and fold increases in the relative densitometric values of PGC-1α and PGC-1β in hearts. c Representative Western blots of whole lysates and fold increases in the relative densitometric values of estrogen-related receptor alpha (ERRα), nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in hearts. OVX ovariectomy, AAC abdominal aortic constriction group; OVX + AAC + Pue, puerarin injected-OVX + AAC group; OVX + AAC + E2, E2 injected-OVX + AAC group; OVX + AAC + Fen, fenofibrate injected-OVX + AAC group. Data are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA coupled with Tukey’s multiple comparison post hoc test. ^*P < 0.05 vs. sham group; ^#P < 0.05 vs. OVX + AAC group; representative Western blots and the densitometric analysis from three independent experiments