Puerarin improves mitochondrial function in Ang II-treated cardiomyocytes. a Real-time analysis of the oxygen consumption rate (OCR) was performed in response to oligomycin A (OLI, 2 μM), followed by carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP, 1 μM) and rotenone/antimycin A (Rot/ant-A; 0.5 μM) in cardiomyocytes subjected to different treatments, as indicated. Representative graph from two independent experiments (n = 4 wells/group/experiment). b–f Nonmitochondrial respiration (b), basal respiration (c), ATP turnover (d), respiration capacity (e), and leakage (f) in NRCMs under different treatments. Data are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA coupled with Tukey’s multiple comparison post hoc test. *P < 0.05 vs. Con group; #P < 0.05 vs. Ang II group; data are representative of two independent experiments