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. 2021 Feb 16;12:601253. doi: 10.3389/fmicb.2021.601253

FIGURE 1.

FIGURE 1

The workflow of the experiment design. Neonates hatched from the eggs were fed 70% moisture Gainesville diet until 10 days old then partitioned into 1,000 larvae per lot for an additional 5-day feeding for acclimation. On the 15th day larvae and frass were further split into two treatments of 500 larvae with equal amount of the frass each. One cup represented the control (Fed) group, in which the larvae were being fed daily (20 g, 2 h prior to the sampling, and 30 g after the sampling), until the end of the experiment. The other cup represented the Starved treatment group, in which the larvae were no longer receiving any food for the rest of the experiment. Five subsamples were taken from each replicate and subjected to RNA isolation and library preparation for shotgun sequencing.