Fig. 4.

Paper size and culture volume optimization. a 16s r-DNA: lane 1—100 bp ladder, lane 2—colony PCR, lane 3—1 mm × 1 mm size ppr with 2 μL CS, lane 4—3 mm × 3 mm size ppr with 3 μL CS, lane 5—5 mm × 5 mm size ppr with 5 μL CS, lane 6—negative control. b β-galactocidase lane 1—100 bp ladder, lane 2—colony PCR, lane 3—1 mm × 1 mm size ppr with 2 μL CS, lane 4—3 mm × 3 mm size ppr with 3 μL CS, lane 5—5 mm × 5 mm size ppr with 5 μL CS, lane 6—negative control. c ITS lane 1—100 bp ladder, lane 2—colony PCR, lane 3—1 mm × 1 mm size ppr with 2 μL CS, lane 4—3 mm × 3 mm size ppr with 3 μL CS, lane 5—5 mm × 5 mm size ppr with 5 μL CS, lane 6—negative control