Table 3.

Results of field and post-harvest phenotyping for pod shattering for 1197 BC₄/F₄ ILs

Phenotype score	Phenotype evaluation	Phenotype description	No. of BC4/F4 ILs
			Effective	After filtering
0	Field	Plant with no shattered pods (indehiscent)	326	311
1	Field	Plant with at least one shattered pod (dehiscent)	866	859
Total	Field		1192	1170
0	Post-harvest	Extreme indehiscent pods which do not open along sutures (putative indehiscent)	52	51
1	Post-harvest	Pods that hardly open along the sutures (putative indehiscent)	181	179
1.5	Post-harvest	Intermediate phenotype between classes 1 and 2 (intermediate)	27	27
2	Post-harvest	Pods which can be opened along the sutures (putative dehiscent)	666	657
3	Post-harvest	Extreme dehiscent pods which open easily, producing a snap/twist when subjected to a slight pressure (high shattering)	266	266
Total	Post-harvest		1192	1180
0	Combined field+post-harvest (Sh y/n)	Indehiscent plant	243	238
0.5	Combined field+post-harvest (Sh y/n)	Intermediate plant	13	11
1	Combined field+post-harvest (Sh y/n)	Dehiscent plant	940	927
Total	Combined field+post-harvest (Sh y/n)		1196	1176

Field and post-harvest data were combined, and a new comprehensive score was adopted (Sh y/n) for the evaluation of pod shattering. For BC₄/F₄ ILs, the ‘effective’ numbers are those for which phenotypic data were acquired, and the ‘after filtering’ numbers are those for plants without 100% accurate data (e.g. few evaluable pods, disease).