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. 2021 Mar 1;11:4907. doi: 10.1038/s41598-021-84039-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Attachment and osteoblastic commitment of hBM-MSCs cultured in contact with the particular bone graft or the bone paste. (a) Representative confocal images of stained hBM-MSCs. Red (phalloidin-AF568): F-actin, blue (Hoechst): nucleus. The particular bone graft and the bone paste were visible as a result of their blue auto fluorescence. Scale bars: 100 µm. (b) Quantification of the total DNA in hBM-MSCs in contact with the particular bone graft or the bone paste. (c,d) SEM images and ALP activity in lysates of hBM-MSCs grown in contact with the particular bone graft or bone paste with or without osteogenic factors for 14 and 21 days. Scale bars: 20 µm. The results are expressed as means ± the SD (N = 3, n = 3; *p < 0.05, **p < 0.01 ***p < 0.001).