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. 2021 Mar 1;11:4907. doi: 10.1038/s41598-021-84039-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rat calvaria healing at 3 and 7 weeks after surgery in a critical size defect model. Representative images of the defects with Movat’s pentachrome staining of undecalcified 7-µm thick frontal section, 3 (a) or 7 (b) weeks after the surgery. The control represents the unfilled defects. Scale bars: 250 µm for the full defects, 100 µm for the high-magnification image (dashed square). Dark yellow: collagen in the mature bone, light red: cytoplasms, dark red: osteoid borders, brown: nuclei, ft: fibrous tissue, oc: osteocytes, nb: newly formed bone, vs: blood vessels, gr: grafts.