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. 2021 Mar 1;6:10. doi: 10.1038/s41536-021-00120-8

Fig. 3. Lineage tracing analysis of S100β-Cre(ERT2)-tdT marked cells following iatrogenic flow restriction for 21 days.

Fig. 3

a–c Verhoeff-Van Gieson stained sections and morphometric analysis of adventitial, medial, intimal and luminal volumes of the b contralateral RCA control compared to the c LCA following ligation for 21d. Data are the mean ± SEM of 10 images, #p < 0.05 vs sham controls. d–f Representative confocal fluorescence images of DAPI nuclei (blue) and S100β-tdT (red) cells in mice treated with corn oil (control) or tamoxifen for 7 days prior to washout for 1 week in d sham and e, f ligated LCA. Data are minimum of four animals per experimental group. Scale bar = 20 µm. Arrows highlight S100β-tdT (red) marked cells. g Representative confocal fluorescence images of DAPI nuclei (blue), immunofluorescence of anti-tdT (red) and anti-Sca1 (far red, white) expression in the contralateral RCA and LCA 21 days post-ligation. Scale bar = 20 µm. h–j Cumulative analysis of the fraction of S100β-tdT+ cells in the h RCA and i LCA and j Sca1/S100β double stained cells in the adventitial, medial and intimal layer of RCA and LCA vessels, respectively, after 21 days in S100β-CreERT2-tdT mice.