Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 1;12:1351. doi: 10.1038/s41467-021-21590-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Quiescent RPE1 cells were immunolabeled by eIF4A3 and Y14 (a). Quiescent cells transfected with indicated siRNAs (h) and permeabilized quiescent cells incubated with RNAse A or not (k) were stained for eIF4A3. poly-glutamylated tubulin (PolyGlu-Tub) antibody stains primary cilia and centrioles (a, h, k) and FOP antibody labels centrosomes (h, k). Nuclei were stained by Hoechst. Lower (a, h) or right panels (k) show enlarged images of the white dashed square in upper (a, h) or left panel (k). b and c are enlarged images from red and yellow dashed squares in lower panel (a). Scale bars in upper (a, h) or left (k) panels and lower (a, h) or right panels (k) panels are 10 and 3 μm, respectively. Relative fluorescence intensity of eIF4A3 and Y14 along the lines were plotted (b, c). Average fluorescence intensity on the line is set to 1.0. Colocalization of eIF4A3 and Y14 was analyzed in 2 μm circles around centrosomes and nuclear speckles and plotted (d, perfect colocalization is set to 1.0). siRNAs were validated by either Western blotting or RT-qPCR. Relative protein (e) or RNA level (f, g) normalized by GAPDH is depicted (average values of siCtrl is set to 1.0). Relative fluorescence intensities of eIF4A3 in the nucleus (i) and those around centrosome (j, l) were measured as described in Fig. 1 legend [the average fluorescence intensity of eIF4A3 in siCtrl (i, j) or buffer (l) condition is set to 1.0]. Columns and bars depict mean ± SD of three independent experiments (f, g). Boxes represent values between the 25th lower and 75th higher percentile, and the red lines mark the median. Whiskers above and below correspond to 0.35th lower and 99.65th higher percentile, respectively (d, i, j, l). n.s P > 0.05, *P ≤ 0.05, ***P ≤ 0.001, and ****P ≤ 0.0001, two tailed Mann–Whitney test (d, i, j, l) and two-tailed t-test (f, g). The number of cells analyzed in three independent experiments is provided (d, i, j, l). Source data are available in a Source Data file.